PLANT TISSUE CULTURE
Tissue culture is the culture and maintenance of plant cells or organs in sterile, nutritionally and environmentally supportive conditions (in vitro).
The basic principle of tissue culture is the totipotencyof cells
Totipotency: The ability of undifferentiated plant tissues to differentiate into functional plants when cultured in vitro
The main aspect of a tissue culture laboratory is the maintenance of aseptic working environment so as to avoid the contamination of growing cultures with microorganisms like bacteria, fungi, and their spores
1.Washing and storage of glassware, plastic ware and other lab wares
2.Preparation, sterilization and storage of nutrient media
3.Aseptic manipulation of plant material
4.Maintenance of cultures under controlled conditions of temperature, light and humidity.
5.Hardening of in vitro developed plants
Steps of plant tissue culture
Selection of Explants
Preparation of Nutrient Media
Sterilization of explants
Inoculation in a nutrient media
Callus induction (culturing)
Sub culturing
Shooting media
Rooting media
Plant regeneration and transfer to sterile soil (green house)
Plant regeneration and transfer to non sterile soil
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